A revolver holds six deadly bullets. Likewise, the train to Sunshine Hill holds Lola, Alice, Janette, Helen, Caroline and Margaret. Margaret is the daughter of a famous sheriff, Caroline is a young girl forced to defend herself on her own, Helen is a confident saloon singer, Janette is a caring doctor, Alice is as wealthy as she is deadly, and Lola leads them with her perfect marksmanship. The find themselves on a train escorting a man named Spencer from the frontier. He counts among his enemies an outlaw gang of notorious robbers. They're more than ready to take his head along with the train’s cache of gold if the girls can’t stop them!